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The mobile phase was isocratically pumped at a flow rate of 1 mL/min. The analytical column Luna C18 (5 ?m, 250 x 4.6 mm ID, Phenomenex, USA) fitted with a refillable guard column (Upchurch Scientific, Oak Harbour, WA, USA) packed with Perisorb RP-18 (30-40 ?m, pellicular) was used for chromatographic separation.

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Jan 25, 2012·If mosquitoes dey bite you every night, Dey fly for your ear like without fear or regards, Then you need?! Raid mosquito coil. 2 for twenty naira, burns slow, very effective, kills mosquitoes and other flying insects and lasts for 8 hrs. DISCLAIMER: IF MOSQUITOES CONTINUE TO BITE YOU AFTER 3 MONTHS, PLEASE PACK OUT.